Traumatic mind harm has turn into a severe public well being downside. Well timed detection, analysis and remedy of mind harm are carefully associated to the prognosis of sufferers, so identification of extremely delicate and particular biochemical markers of mind harm has essential scientific worth. At present, probably the most studied and most promising marker is the protein S100B. On this research, a speedy quantitative biosensor for S100B was established utilizing colloidal gold labeling and double antibody (8C10-6B8) sandwich immunochromatography. The biosensor was able to quantifying S100B inside 15 min, and confirmed no cross-reactivity with S100A, NSE, GFAP, or PGP9.5. The detection restrict was decided to be 4.6 pg mL−1 with a linear vary of 0.01–2 ng mL−1. Restoration experiments additionally indicated that the strategy had a suitable accuracy. Furthermore, the quantitative colloidal gold assay correlated nicely with the outcomes of a chemiluminescence immunoassay when testing 40 scientific serum samples. Our developed colloidal gold quantitative immunochromatographic biosensor is a speedy, delicate, particular and correct methodology for the detection of S100B protein in serum, which is helpful within the clinic for early analysis, in addition to evaluation of illness development and prognosis of traumatic mind harm.