We current the event of a health-monitoring nanofluidic membrane using biocompatible and biodegradable graphene oxide, chitosan, and graphene quantum dots. The nanoconfinement offered by graphene oxide nanolayers encapsulates chitosan molecules, permitting for his or her conformational modifications and switchable hydrophobic–hydrophilic conduct in response to pH variations. This low-dimensional membrane operates as an array of nanofluidic channels that may launch quantum dots upon pH change. The photoluminescence emission from quantum dots permits fast and dependable optical visualization of pH modifications, facilitating environment friendly human well being monitoring. To make sure fouling prevention and allow a number of usages, we undertake a design strategy that avoids direct contact between biomarkers and the nanochannels. This design technique, coupled with good mechanical properties (Younger’s modulus of 5.5 ± 0.7 GPa), preserves the integrity and performance of the sensors for repeated sensing cycles. Moreover, leveraging the reminiscence impact, our sensors will be reloaded with graphene quantum dots a number of instances with out vital lack of selectivity, attaining reusability. The wide-ranging capabilities of 2D supplies and stimuli-responsive polymers empower our sustainable strategy to designing low-dimensional, strong, and versatile sensing supplies. This strategy permits for the combination of assorted biorecognition parts and sign transduction modes, increasing the flexibility and purposes of the designed supplies.